Services
SEC Column Phase Screening Service
| Sample: | Monoclonal antibody F, MW 145 kD, pI=8.4 |
| Goal: | In search of high resolution separation between protein monomer, aggregates, and fragments. |
| Approach: | Screen different column phases (Zenix-300 and Zenix-C 300) to achieve best separation. |
| Conclusion: | Zenix-C SEC-300 (3 µm, 300 Å) column delivers baseline separation of monoclonal antibody monomer and aggregates. |
MAb F Analysis on Zenix-300 and Zenix-C SEC-300
Column: Zenix-300, Zenix-C 300(3 µm, 300 Å, 7.8 x 300 mm )
Mobile phase: 150 mM Sodium Phosphate, pH 7.0
Flow rate: 1.0 mL/min
Detector: UV 280nm
Column temperature: 25 °C
Pressure: 74 bar
Injection volume: 10 µL
Sample: 1.23 mg/mL MAb F in 10 mM sodium succinate, pH 5.0