Services
SEC Mobile Phase Screening Service
| Sample: | CHO cell expressed Fc Fusion Protein, 160 kD |
| Goal: | In search of high resolution separation between protein monomer, dimer, and high molecular weight aggregates. |
| Approach: | Screen different mobile phase to achieve best separation and sample recovery. |
| Conclusion: | Zenix SEC-300 (3 µm, 300 Å) column delivers baseline separation of Fc fusion protein, its oligomer, and impurity. With the addition of arginine, the hydrophobic interaction between Fc fusion protein and column resin is minimized. |
CHO cell expressedFc Fusion Protein Analysis on Zenix-300
Column: Zenix-300(3 ym,300 A 7.8 x 300 mm
Mobile phase 1: 150 mM Phosphate buffer, pH 7.0
Mobile phase 2: 50mM phosphate buffer, 150 mM NaCI, 27 mM L-Arginine in 1 LH20,adjust to pH7.0 with HCI
Flow rate: 0.5 mLmin; Detector.UV 280 nm
Columntemperature: Roomtemperature
Injectionvolume: 50 pL
Pressure: 50 bar
Sample: Fc-fusion protein 160kD(2.0 mgmL)