Resin Technologies

We have established three platforms of base-bead resin technology: Silica, Polystyrene/Divinylbenzene (PS/DVB) and Polymethacrylate (PMA) with well-controlled particle sizes ranging from 1.7 to 100 µm and pore sizes from non-porous to 2000 Å. On the base beads, a broad spectrum of innovative surface modifications has been developed to meet the ever-advancing customer needs for chromatography analyses and separations.

Silica-based Resin Technology

The integration of Sepax's silica technology and surface chemistry innovation has enabled multiple product lines, including reversed phased (RP), ion-exchange (IEX), and size exclusion chromatography (SEC) resins for small and biological molecules. Our market-leading SEC product line includes Unix ®, Unix ® -C, Zenix ®, Zenix ® -C, SRT ®, SRT ® -C, SRT ® -10, and Nanofilm ® . These products have particle size selection of 1.8, 3, 5 and 10 µm and pore size selection of 80, 100, 150, 300, 500, 1000 and 2000 Å.

PS/DVB-based Resin Technology

Sepax's non-porous PS/DVB particle technology offers particle size selection of 1.7, 3, 5 and 10 µm. The non-porous particles have unique properties of fast mass transfer and minimized lateral diffusion that enable high resolution separation. The integration of non-porous PS/DVB beads and innovative surface chemistries has led to Proteomix® ion-exchangers, Proteomix® HIC and Antibodix™ WCX product lines. Proteomix® SAX, WAX, SCX and WCX offer a complete solution for ion-exchange separation of biological molecules. Antibodix™ WCX is specially designed for separation and analysis of monoclonal antibodies. Proteomix HIC series are ideal for hydrophobic interaction separation of biomolecules, such as ADC samples. Among these products, 1.7 µm particle is a leading technology for achieving highest efficiency separation.

Sepax's porous PS/DVB particle technology has enabled the development of our Proteomix® RP and PolyRP™ products for hydrophobic and reversed phase analyses and separations with well-controlled particle sizes of 3, 5, 10, 15, 30 and 60 µm and pore sizes of 100, 300, 500 and 1000 Å. Our monosized PS/DVB base bead technology leads to the synthesis of PMA particles of narrow size distributions (D90/D10 ≤ 1.3). PolyRP™ is a polymeric revered phase bulk resin, highly stable over a variety of operational conditions. Compared to silica-based reversed phase resins, PolyRP™ is stable at extreme pH (1- 14) with a similar separation efficiency and better selectivity. They are stable to resist high temperatures up to 200 °C (operating temperature up to 80 °C), and compatible with many commonly used organic solvents and aqueous buffers.

Sepax's low cross-linking PS/DVB particle technology offer the particle sizes of 5 and 10 µm with cross-linking degree of 5, 8 and 10%. By functionalizing the low cross-linking PS/DVB particles with sulfonic acid (-SO3H), followed by chelating of metal ions, Carbomix® product lines have offered our customers advantages in carbohydrate separation.

PMA-based Resin Technology

Sepax's Monomix™ PMA particle technology has produced resin base beads of highly uniform sizes (D90/D10 ≤ 1.3) with particle sizes of 10, 15, 30 and 60 µm and pore sizes of 500 and 1000 Å. The integration of the Monomix™ PMA particle technology and innovative surface chemistries has led to Monomix™ MC SEC size exclusion chromatography resin product line and Monomix™ IEX ion-exchange chromatography resin product line. Monomix™ MC SEC resins have proprietary hydrophilic surface functional groups that minimize non-specific binding. Key benefits of this product line include: highly stable over a variety of operational conditions, broad pH tolerant range (1-14) (an advantage over silica-based SEC resins), elevated operating temperature (up to 80°C), high operating pressure (up to 20 bar), high theoretical plate number, smooth surface and highly spherical particles (easy column packing), compatible with many commonly used organic solvents and aqueous buffers. Compared to agarose-based resins, Sepax Monomix™ MC SEC resins have stronger base beads and can resist higher column back pressure and higher operation flow rate. Additionally cleaning and CIP are user friendly and effective, thus prolonging resin life, increasing purification productivity and reducing purification cost. Monomix™ MC SEC resins have demonstrated performance in the separation of proteins, polysaccharides, VLP, and other biomacromolecules.

Sepax Monomix™ IEX ion-exchange chromatography resins enable the separation of native biological samples without disruption of high order structures. They have been widely used in the analysis and separation of ionizable pharmaceutical molecules. Currently commercially available IEX chromatography resins are mostly based on soft agarose or polymethacrylate particles, which only accommodates low pressure operation. Sepax has developed the alternative polymethacrylate-based ion exchange media that can stand higher operation flow rates.